King_Marmalade
King_Marmalade t1_ixlyb1c wrote
Reply to comment by SemogAziul in If freezing tissue generally damages the cells, how are we able to freeze human eggs and embryos for birthing later? by badblackguy
The cell lines I've generated and distributed to ATCC were all frozen in DMSO (which you mentioned) in fetal bovine serum. For me this included cell types from many tissues like skin, lung, liver, endothelial cells (from vascular tissue), etc. Even poorly differentiated glial cells are frozen in DMSO, but using a culture medium in place of serum so they don't differentiate.
King_Marmalade t1_iu62syp wrote
Reply to comment by Snufflepuffster in Major breakthrough in cancer research: Papers reveal 'dark matter' that contributes to disease's growth by Ezekiel_W
This is a big shift that occurred decades ago
King_Marmalade t1_ixnl66s wrote
Reply to comment by Lowtiercomputer in If freezing tissue generally damages the cells, how are we able to freeze human eggs and embryos for birthing later? by badblackguy
Glial cells encompass multiple types of mature cells with diverse functions (astrocytes, microglial cells, oligodendrocytes) you can think of them as sort of "helper cells" for neurons. In some types of cancers, cells can enter what is considered a "poorly differentiated" state, where they lose some characteristics of a mature cell and become more stem-like. Some of the cell lines our lab generated were from GBM (glialblastoma multiforme) samples.