Jordanno99 t1_jb6leae wrote on March 6, 2023 at 8:41 PM Reply to Why don't researchers just use conjugated primary antibodies for ELISAs? by ursoda Often you are interested in a primary antibody that binds the antigen. Depending on where your primary antibody is from (serum, plasma, expression) it may not have a tag for direct detection, and it would be cumbersome to chemically add one. Permalink 2
Jordanno99 t1_irdxll2 wrote on October 7, 2022 at 9:37 AM Reply to comment by Mikedc1 in is it possible to synthesize proteins chemically? by yeeturking You normally add a tag such as ctag, histag, monoFc or biotin to assist with purification. Permalink Parent 3
Jordanno99 t1_jb6leae wrote
Reply to Why don't researchers just use conjugated primary antibodies for ELISAs? by ursoda
Often you are interested in a primary antibody that binds the antigen. Depending on where your primary antibody is from (serum, plasma, expression) it may not have a tag for direct detection, and it would be cumbersome to chemically add one.