>some remote desert, or arctic, region on Earth before trying it on Mars or the moon?

Yes, which is why various organizations have tried various endurance projects to see what happens. The ill fated biospheres immediately jump to mind. There is this one: https://www.hi-seas.org/

When combining the results of them with economic reality and the willful ignorance of most humans, one can easily jump to the bad news: Humanity will not be colonizing anything.