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Bryan_Waters t1_j056jai wrote

Not sure if OP was involved in conducting the study, but I’m curious why they decided to fix the tissue in 4% neutral buffered formalin and not 10% which is typically standard practice. Underfixation of tissue can lead to false negative staining in IHC, so sort of curious what the rationale was behind that decision.

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Skylark7 t1_j05drzm wrote

I've seen standard histology fixation solution referred to as 4% because it's ~4% w/v of formaldehyde. The 10% is v/v.

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clayeos t1_j070jzt wrote

I wish I was more smarter like you guys :/

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StateOfContusion t1_j07fgse wrote

Not necessarily smarter, just better educated in their fields.

I'm going to hire an electrician to replace my breaker panel. Is he smarter than me? In his field of expertise, 100% he is. Could I learn it if I dedicated 5 years or so? Absolutely.

Just don't ask me to learn and understand quantum mechanics. That's probably outside of my realm.

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clayeos t1_j07t2hu wrote

This is very true. Thank you. I appreciate that.

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Gwtheyrn t1_j08c437 wrote

Anyone who says they understand quantum mechanics doesn't.

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Bakemono30 t1_j08qbjd wrote

I don't understand quantum mechanics

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garbage-pale-kid t1_j090pvi wrote

I tend to trust people more when they say "I get the gist of it".

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Gwtheyrn t1_j09og8p wrote

Yeah, I understand some concepts about "this is the way things are."in rough, layman's terms, but not the how's and whys.

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nsfbr11 t1_j0960k8 wrote

Just not true. What is true is that quantum mechanics has limits.

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Baseball_bossman t1_j09111q wrote

“ If you think you understand quantum mechanics, you don’t understand quantum mechanics.” - Richard P. Fleynmen

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popejubal t1_j09jebm wrote

I read all of these comments and I’m fairly certain that most of what I read was definitely words. That’s about as far as I got.

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Otherwise-Ad4895 t1_j078o5i wrote

Dont trip, it's just jargon. Look up words you don't understand. Repeat forever.

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clayeos t1_j07azv7 wrote

Wow thanks man! You’re a nice person.

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Skylark7 t1_j0826t3 wrote

It just means we've had the misfortune to be in a lab reeking of formalin. You're not missing out on anything meaningful or fun. :-)

And as other folks have said, it's just jargon. The first person said the heart tissue may not have been preserved in a standard way and that could impact the results. I'm pointing out that there are some less-common ways to describe the chemical used to preserve the tissue. We don't know which is right, whether they did something unusual or used very unusual language.

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clayeos t1_j08clz1 wrote

You guys are all so nice! Thank you! Honestly you guys have made my day.

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thetransportedman t1_j08sjlk wrote

This is why it’s standard practice to have scientists very similar to your work be the ones reviewing your papers for submission. It’s minutiae you wouldn’t know unless you also do the same type of protocol. You’ll notice it in grad school journal clubs. The best critique are people doing similar things while others who are equally talented can only ask bigger picture questions

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sjk4x4 t1_j09efgd wrote

I dated a woman for a while that had a p.h.d. She used it working for a college to translate papers written by faculty into language they could use to teach to students.

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Blah-squared t1_j09bzwc wrote

Idiot… it’s not “more smarter”…

It’s ”smarterer”…

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popformulas t1_j08yetm wrote

Hey pal, it’s just me and you over here with cheetoh fingers

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LilyGreen347 t1_j0989yo wrote

The hardest thing to teach is the desire to learn. Everything else just takes time and patience.

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shelleyflower77 t1_j08086w wrote

I was going to say the same until I read the comment below. Thanks.

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Bryan_Waters t1_j05ffrq wrote

That’s interesting, is this outside of the US? I’ve never seen it indicated this way in a Pathology report or in a lab SOP, in or out of the US.

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chem199 t1_j07gixn wrote

It is German, not sure if that changes anything.

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Skylark7 t1_j07guqx wrote

I've seen it in papers from people fixing tissue culture cells or animal tissues. Also I don't think fixation in 4% v/v would even crosslink well enough to section and stain. Their photomicrographs looked pretty typical for FFPE.

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ducklingsaver t1_j06pdwh wrote

Most likely an error in methods reporting. I’m guessing some mixup since most labs I know will either use 10% NBF or 4% PFA for human tissue. Either way, the example photos do not appear under fixed.

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