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basmwklz OP t1_iw6aqnq wrote

Abstract: >Background: Typical vivarium temperatures (20-26°C) induce facultative thermogenesis in mice, a process attributable in part to uncoupling protein-1 (UCP1). The impact of modest changes in housing temperature on whole body and adipose tissue energetics in mice remains unclear. Here, we determined the effects of transitioning mice from 24°C to 30°C on total energy expenditure and adipose tissue protein signatures. Methods: C57BL/6J mice were housed at 24°C for two weeks and then either remained at 24°C (n=16 per group, 8M/8F) or were transitioned to 30°C (n=16 per group, 8M/8F) for 4 weeks. Total energy expenditure and its components were determined by indirect calorimetry. Interscapular brown adipose tissue (iBAT) and inguinal white adipose tissue (iWAT) proteins were quantified by western blot and quantitative proteomics. Results: Transitioning from 24°C to 30°C reduced total energy expenditure in both male (-25%) and female (-16%) mice, which was attributable to 36% and 40% decreases in basal energy expenditure in males and females, respectively. Total iBAT UCP1 protein content was 50% lower at 30°C compared to 24°C, whereas iWAT UCP1 protein content was similar between conditions. iBAT UCP1 protein content remained 20-fold greater than iWAT at 30°C. 183 and 41 proteins were differentially expressed between 24°C and 30°C in iBAT and iWAT, respectively. 257 iWAT proteins differentially expressed between sexes at 30°C were not differentially expressed at 24°C. Summary: 30°C housing lowers total energy expenditure of mice when compared to an ambient temperature (24°C) that falls within the National Research Council's guidelines for housing laboratory mice. Lower iBAT UCP1 content accompanied chronic housing at 30°C. Further, housing temperature influences sexual dimorphism in the iWAT proteome. These data have implications regarding the optimization of preclinical models of human disease.

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JoeDoherty_Music t1_iw6dn38 wrote

Does anyone have this in English, I don't speak Turbo Encabulator

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SkomerIsland t1_iw6tmbm wrote

Shivering uses more energy

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grewapair t1_iw76psp wrote

The mice were either held at 75 degrees or 86. They weren't shivering in either case.

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DependentPangolin911 t1_iw79m38 wrote

Thermoneutral zone for mice is around 30 deg C, so they may well have been at 75 deg. There’s a long standing argument that mouse rooms are kept too cold for mice for the comfort of humans, which is likely impacting their behavior and experimental results.

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BafangFan t1_iw6lf5q wrote

Brown adipose tissue is a type of fat that burns fat to generate body heat.

By raising the room temperature by about 12f, rats burned 16-25% less energy than rats kept at the lower temp.

But 12f is the difference between 60 degrees and 72 degrees, and I think we can all argue that 60 degrees is a pretty miserable temp.

To critique the point of the study, people aren't fat because their room is too warm. Look at people in Thailand, India, Vietnam. It's hot as balls over there, and many don't have A/C - and yet those are typically rather lean countries.

On the flip side, NASA astronaut Ray Cronise lost 80 pounds in part through sleeping in shirt and shorts with the window open all year long.

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[deleted] t1_iw6o35p wrote

[deleted]

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malppy t1_iw78mgj wrote

If a study was done it'd probably be attributed to caloric intake and diet. The portion sizes in SEA are super small compared to the west. Northern SEA countries also consume a high amount of veggies in their diet.

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oMaddiganGames t1_iw6rrsz wrote

My wife would ague 60f is still too hot for night time.

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myurr t1_iw6uv8d wrote

And yet she's a block of ice when first getting into bed, leaching your very soul out of your body to give her life again when her freezing feet touch your legs.

Five minutes later and she's radiating more heat than Chernobyl's core.

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RainingTenebres t1_iw6whr2 wrote

Welp I'm not the only one. Makes me feel better to know it's not just me doing this to my partner.

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Thomas_the_chemist t1_iw6xhjv wrote

But the temp wasn't that low. 24C is 75F and they went up to 30C which is 86F. Both temps are relatively warm. I wonder why the study didn't move a third group to a colder chamber, like 18C?

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Zerothian t1_iw6zplm wrote

>and I think we can all argue that 60 degrees is a pretty miserable temp.

Honestly, not really, at least not for everybody. I'm in the UK and that's roughly my ambient in the living room. This is measured by a sensor in my computer case at idle and showing 18c, so realistically it'd be a couple degrees cooler outside the case. It's fine as long as I'm fully clothed, it is pretty miserable if I'm not wearing sleeves and socks though.

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JohnAStark t1_iw73iea wrote

We love our house at a nice low temp - 58F at night and 60-62F during the day. Of course it helps that we are cheap!

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D4ltaOne t1_iw7stl5 wrote

Thats... Not the point of the study tho. The background says that they wanted to see the effect raising room temperature has.

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Boost_Attic_t t1_iw6gk6p wrote

Sigh...

I tried to read the whole comment, but I just kept feeling more and more stupid...

Edit. But also I think it says warmer = less energy expended

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love2Vax t1_iw7oeek wrote

We use mice as an animal model for medical research before we test the same thing on humans. The current recommendations is to house them at 24C. This experient was questioning how housing them at a warmer temperature might impact the results of the experiments we do before human trials. Questions about how a new drug is metabolized in mice before we test it on humans can have big implications if we aren't testing the mice under the correct environmental conditions.

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Gespuis t1_iw75y1y wrote

30 degrees? With these energy prices?!

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