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Lepmuru t1_j36hlia wrote

You are 100% accurate with your assessment. The only thing keeping you from understanding is an assumption you made involuntarily (which also is kind of non-trivial).

You select for neomycin-resistance, but not by adding neomycin. Instead, to select eukaryotic cells you would use a substance called geneticin or G418 which is toxic to eukaryotic cells, but cleared by the neomycin-resistance gene.

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Casvanas OP t1_j37i941 wrote

Thank you so much, that really clears things up!

This image from my textbook is what confused me. I assume they said they just said neomycin was added for the sake of simplicity?

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Lepmuru t1_j37jpqr wrote

Might simply be an error in the print or result from oversimplification. Happens even with the most reviewed books and manuscripts.

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