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-Metacelsus- t1_izs5kev wrote

Reply to comment by sometimesgoodadvice in Does a plasmid with several ORF coding for several proteins get transcribed to a single or multiple rna transcripts? What determines the start stop signal for rna transcription? by kathmkath

> because the tRNA that are complementary to the stop codons are not charged with amino acids.

This is not correct, stop codons are recognized by release factors not tRNAs.

Otherwise a very good answer though.

I should mention, when I want to express multiple eukaryotic proteins from the same promoter I will use 2A peptides to separate them. For example: https://www.nature.com/articles/s41598-017-02460-2

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