Metalmind123 t1_ixmtm0c wrote
Reply to comment by Magnetic_Syncopation in If freezing tissue generally damages the cells, how are we able to freeze human eggs and embryos for birthing later? by badblackguy
That is one option, though the type of sugar matters.
As does the temperature you intend to freeze it at. Be it liquid nitrogen storage, a -80°C freezer, or your bog standard -20°C.
Common preservants would be DMSO, Glycerol or Trehalose (less used because of the cost), combined with appropriate media and buffer. In general, these together manage ice crystal formation (DMSO, Glycerol, Trealose), keep the pH in the right range (buffer), and reduce oxidation (DMSO).
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