Westbrook_Level t1_ixmig0i wrote
Reply to comment by Magnetic_Syncopation in If freezing tissue generally damages the cells, how are we able to freeze human eggs and embryos for birthing later? by badblackguy
We almost always use 5% DMSO. It works best in my experience.
https://en.wikipedia.org/wiki/Dimethyl_sulfoxide
Then frozen slowly in vials in something like this.
https://www.thermofisher.com/order/catalog/product/5100-0036
When thawing you went a fast thawing process so putting the vial in a water bath at body temp works well.
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