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King_Marmalade t1_ixlyb1c wrote

Reply to comment by SemogAziul in If freezing tissue generally damages the cells, how are we able to freeze human eggs and embryos for birthing later? by badblackguy

The cell lines I've generated and distributed to ATCC were all frozen in DMSO (which you mentioned) in fetal bovine serum. For me this included cell types from many tissues like skin, lung, liver, endothelial cells (from vascular tissue), etc. Even poorly differentiated glial cells are frozen in DMSO, but using a culture medium in place of serum so they don't differentiate.

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Lowtiercomputer t1_ixmop80 wrote

What is a differentiated glial cell?

Glial cells make the myelin sheath, right?

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Bax_Cadarn t1_ixmqkic wrote

I don't know what makes poorly differenciated glial cells any special but yes, gliala cells makes myelin sheath, as well as supporting cns tissues, and differenciation is the process of a cell turning from a basic, undifferenciated cell into one with a very specific function.

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Neurofish8 t1_ixn0sfn wrote

Depends on the glial cell: oligodendrocytes produce myelin in the central nervous system, but there are also microglia and astrocytes. Peripheral nervous system has Schwann cells that produce myelin.

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King_Marmalade t1_ixnl66s wrote

Glial cells encompass multiple types of mature cells with diverse functions (astrocytes, microglial cells, oligodendrocytes) you can think of them as sort of "helper cells" for neurons. In some types of cancers, cells can enter what is considered a "poorly differentiated" state, where they lose some characteristics of a mature cell and become more stem-like. Some of the cell lines our lab generated were from GBM (glialblastoma multiforme) samples.

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Supersnow845 t1_ixo131r wrote

All cell work I do uses DMSO and fetal bovine serum as well, it’s the standard for freezing cells down in -80 freezers

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