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newappeal t1_isbbnbh wrote

Reply to comment by regular_modern_girl in Why do people take iodine pills for radiation exposure? by Furrypocketpussy

>Is that really biological systems distinguishing, though, or is that just human researchers looking at isotope ratios and using them to determine where a given element in a biochemical context came from?

Those are the same thing. The biochemical discrimination is the mechanism that causes the difference in isotope ratios.

However, if you're asking whether the discrimination is teleological in nature - i.e. that it has a biological "purpose" that has been acted upon by selection pressure - then the answer is no, it is not "intentional", but rather a correlate of the differences in photosynthetic strategies that were directly selected for.

Edit: To specifically address the last bit: they're both getting the same atmospheric carbon, just different isotope fractionations of it.

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regular_modern_girl t1_isbetxk wrote

To clarify, I guess what I meant by “distinguish” is whether or not different isotopes behave fundamentally differently as far as biochemistry is concerned (apart from the obvious difference that some are radioactive and give off ionizing radiation that destroys biological macromolecules, etc.), like in the way that different elements do, and the only specific example of that it seems is with different hydrogen isotopes. It seems that basically apart from deuterium, as long as an isotope isn’t radioactive, it can be used biologically just the same as the more common isotope of that element (assuming a biochemically-relevant element here, obviously) without causing any problems.

Although tbf, I guess the comment that started this thread was asking if the human body (in particular the thyroid gland) “knew the difference” between different isotopes of iodine, and I guess sort of what I’m asking here is, do C3 plants take in more or less of a given isotope than C4 plants because something is making a distinction between the isotopes (regardless whether or not there’s a specific “purpose” to taking in more of one than the other), or is it just a side effect of getting carbon from CO2 in the air versus carbonic acid/carbonate in the water?

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newappeal t1_isbi1rp wrote

>To clarify, I guess what I meant by “distinguish” is whether or not different isotopes behave fundamentally differently as far as biochemistry is concerned

I would maintain that if the isotopes are incorporated at different rates (as they indeed are), then they behave differently by definition. I'm not sure what "fundamentally" means here - if you mean "substantially" in the sense of having biological relevance, then I would say no, they do not. But "biological relevance" itself has no objective definition. I could say that they form the same sort of chemical bonds, but that's not actually entirely true, just mostly true, of isotopes.

>or is it just a side effect of getting carbon from CO2 in the air versus carbonic acid/carbonate in the water?

I should specify that CO2 ultimately comes from the atmosphere in both cases. The difference is the carboxylation reaction in C3 plants uses carbon dioxide directly, whereas in C4 plants, CO2 first reacts with water to form bicarbonate before being conjugated to an organic molecule. The relevant factors for fractionation are therefore the diffusion rate of 13CO2 and 12CO2 in the gaseous state, and the preferences for the relevant enzymes for each carbon isotope.

The underlying physical principles are the same here as in the case of neutron-free hydrogen vs. deuterium. The difference is just one of degree. Carbon-13 is 8.3% heavier than Carbon-12, while deuterium is twice as heavy as hydrogen. Moreover, hydrogen atoms (of all isotopes) are commonly transferred between compounds individually, whereas single carbon atoms do not appear in biological reactions. (In the specific case of carbon fixation, the carbon makes up a minority of the mass of the molecule that actually participates in the reaction.) The discrimination between hydrogen and deuterium in chemical and physical processes is therefore as high as it could possibly be for stable isotopes, and the differences in rates between them is therefore maximal compared to other elements. These discrepancies in rates, which differ in relative magnitude direction for different processes, are enough to upset the balance of biological systems if they are supplied with too much deuterated water. However, I also can't say for certain that a biological system supplied with only 13C wouldn't suffer a similar fate. After all, we're comparing the partitioning of naturally-occurring isotopic ratios of stable carbon isotopes to the extreme hypothetical of exposing an organism to pure heavy water.

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