Submitted by ursoda t3_11k1x13 in askscience
a-synuclein t1_jb5w8nw wrote
For ELISAs to show binding we used an HRP-conjugated protein that we conjugated with a kit (3 hour process, very easy to do) and it worked great. Plenty of labs conjugate the "primary" sensor (antibody or protein) and forgo secondary. There are disadvantages of course but sometimes you don't have a choice and it works fine with enough concentration.
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