Submitted by Natolx t3_10yxpkv in askscience
Tiny_Rat t1_j81m6ud wrote
Reply to comment by EmilyU1F984 in Can the Radiation from a Sample of Depleted Uranium Sterilize? by Natolx
If they autoclave it, a sealed vessel will still explode, as the pressure inside the vessel and the machine will still change at different rates. Plus, killing the bacteria won't make the solution less cloudy, it will just be cloudy with dead bacteria instead of living ones. And, worst of all, heat treatment will most likely kill the fluorescent proteins anyways.
[deleted] t1_j839x39 wrote
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RebelWithoutAClue t1_j82asnn wrote
It could be autoclaved in a pressure cooker if the protein can tolerate the temp.
In a pressure cooker the pressure is also exerted on the outside of the ampule. No pressure differential, no kaboom.
[deleted] t1_j82dwcs wrote
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